Infant formula and adult nutritionals - Determination of trans and total (cis + trans) vitamin K1 content - Normal phase HPLC
This document specifies a method for the quantitative determination of trans and total (cis + trans) vitamin K1 in infant, pediatric and adult nutritionals using normal phase (NP) high-performance liquid chromatography (HPLC) with post-column reduction and fluorescence detection. The method demonstrated good linearity over a standard range of ~2 ÎĽg/l to 80 ÎĽg/l trans vitamin K1, and the limit of quantification (LOQ) was estimated to be 0,4 ÎĽg/l for standards and 0,09 ÎĽg/100 g ready to feed (RTF) for samples assuming 4 grams of sample are diluted to 10 ml.
| SDO | ISO: International Organization for Standardization |
| Document Number | ISO 21446 |
| Publication Date | Not Available |
| Language | en - English |
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| Committee | ISO/TC 34 |