ISO 11701:2009

Current Revision
Vegetable fats and oils - Determination of phospholipids content in lecithins by HPLC using a light-scattering detector

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Vegetable fats and oils - Determination of phospholipids content in lecithins by HPLC using a light-scattering detector

ISO 11701:2009 specifies a method for the quantitative determination of phospholipids content by high performance liquid chromatography (HPLC) using a diol column and a light-scattering detector.

The method is applicable to crude, oil-containing lecithins, and to oil-free lecithins and lecithin fractions from vegetable fats and oils.

The method is not applicable to animal and ruminant lecithins and enzymatically hydrolysed lecithins as the peak separation of lysophosphatidylethanolamine (LPE), lysophosphatidylinositol (LPI) and lysophosphatidic acid (LPA) is insufficient.

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